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Home / Proteins & Peptide

UBE2C/UBCH10, His, Human

SKU : BHP18500177

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iXCells Biotechnologies

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Mfr.No.	IX02968, IX02968-1
Description	Human Ubquitin Conjugating Enzyme E2 C (UBE2C)/UBCH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Molecular Weight	Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids (a.a.) of human UBE2C/UBCH10 and 12 a.a. vector sequence including 6 x His tag at N-terminus.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	Human
Endotoxin Level	Less than 1 EU/ug of rHuUBE2C, His as determined by LAL method.

Synonyms	Ubiquitin-conjugating enzyme E2 C, EC 6.3.2.19, Ubiquitin-protein ligase C, Ubiquitin carrier protein C, Ubc10, UBCH10, dJ447F3.2.
Sequence	MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAARGPVGKRLQQE?LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLRYKLSLEFPSG?YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDVRTILLSIQSL?LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQEP

Purity	95 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500177-1	IX02968-1	1mg	$1872		Add to Cart

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