Human Ubiquitin Activating Enzyme (E1) (UBE1), also known as UBA1, GenBank Accession No. NM_003334, a.a. 2-1058(end), with N-terminal FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=119 kDa.
host species: insect
Host species: insect
Host cell line: Sf9
Specific activity: 5 pmole/min/ug
Immunogen Region
2-1058(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 80 ug/ml FLAG peptide.
Species
Human
Application
Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
Assay was done according to UBE1 Inhibitor Screening Assay Kit (Biohippo #79957) with UBE1 titrated from 1500 ng/rxn to 0 ng/rxn.
Avoid freeze/thaw cycles.
GenBank
NM_003334
Mw(kda)
119 kDa
Synonyms
UBE1, UBA1
Uniprot
P22314
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008). 2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).