Human Ubiquitin Activating Enzyme (E1) (UBE1), also known as UBA1, GenBank Accession No. NM_003334, a.a. 2-1058(end) with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 145 kDa.
Specific activity: 1 pmole/min/ug
Immunogen Region
2-1058(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% Glycerol and Glutathione.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done according to UBE1 Inhibitor Screening Assay Kit (Biohippo #79957) with UBE1 titrated from 6000 ng /rxn to 0 ng/rxn.
Avoid freeze/thaw cycles.
GenBank
NM_003334
Mw(kda)
145 kDa
Synonyms
E1, UBA1, Ubiquitin Activating Enzyme
Uniprot
P22314
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Ramser, J., et al., AJHG. 2018;82(1): 188-193. 2. Leidecker, O., et al., Cell Cycle. 2012; 11(6): 1142-1150.