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Home / Proteins & Peptide

UbcH7, His-tag (Sf9-derived)

SKU : BHP18200813

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BPS Bioscience

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Mfr.No.	80318
Description	UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 1-154 (end), with C-terminal His-tag, MW = 18.7 kDa, expressed Sf9 cells via a baculovirus expression system.
Immunogen Region	1-154
Tag	C-terminal His-tag
Formulation	50 mM Tris, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT
Species	Human
Application	Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	50 ul reaction mix(4 mM ATP, 5 mM MgCl2, 1 mM DTT,UBE1 (0.5 ug), UbcH7, Biotin-Ubiquitin(0.5 ug) and Smurf2 (1.5 ug) in 50 mMTris, pH 7.4) for 30 min at 37°C. Add toStreptavidin-coated plates for 30 minat 37°C. Incubate with anti-Smurf2, thenHRP-labeled secondary antibody, andchemiluminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_003347
Mw(kda)	18 kDa

Synonyms	UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3
Uniprot	P68036

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200813	80318	50 ug	$319		Add to Cart

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