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Home / Proteins & Peptide

UbcH7, His-tag (E. coli-derived)

SKU : BHP18200812

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Mfr.No.	80317
Description	UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 2-end, with N-terminal His-tag, MW = 18 kDa, expressed in an E. coli cell expression system.
Immunogen Region	2-end
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	50 ul reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, UBE1 as an E1 (0.5 ug), UbcH7, Biotin-Ubiquitin (0.5 ug) and Smurf2 as an E3(2 ug) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, thenEuropium-labeled secondary antibody, and detect by time resolved fluorescence (lambdaex=330 nm, lambdaem=620 nm). Avoid freeze/thaw cycles.
GenBank	NM_003347
Mw(kda)	18 kDa

Synonyms	UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3
Uniprot	P68036

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Katsanis, N., et al. 1998. Genomics 51: 128- 131.2. Cook, W.J. et al., 1997. Biochemistry 36 (7): 1621-1627.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200812	80317	100 ug	$319		Add to Cart

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