Human UbcH5c, also known as UBE2D3, (GenBank Accession No. NM_001300795), amino-acids 2-end, with N-terminal His tag, MW=18 kDa, expressed in an E. coli expression system.
Immunogen Region
2-end
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 220mM Imidazole, 0.04% Tween20, 20% glycerol
Species
Human
Application
Useful in conjunction with E1 and E3 for the studies of enzyme kinetics, screening inhibitors and selectivity profiling.
Notes
Avoid freeze/thaw cycles.
GenBank
U39318.1
Mw(kda)
18 kDa
Synonyms
ubcH5c, UBE2D3
Uniprot
P61077
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Markson G. et al., Genome Res. 19, 1905-1911 (2009). 2. Nuber U. and Scheffner M., J. Biol. Chem. 274 (11), 7576-7582 (1999).