Human UbcH5a also known as UBE2D2 (GenBank Accession No. U39317.1), amino-acids 2-end, with N-terminal His tag, MW=18 kDa, expressed in an E. coli expression system.
Host cell line: E. coli
Immunogen Region
2-end
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 20% glycerol, 105 mM Imidazole
Species
Human
Application
Useful in conjunction with E1 and E3 for the studies of enzyme kinetics, screening inhibitors and selectivity profiling.
Notes
Avoid freeze/thaw cycles.
GenBank
U39317.1
Mw(kda)
18 kDa
Synonyms
ubcH5c, UBE2D2
Uniprot
P62837
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Markson G. et al., Genome Res. 19, 1905-1911 (2009). 2. Nuber U. and Scheffner M., J. Biol. Chem. 274 (11), 7576-7582 (1999).