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Home / Proteins & Peptide

UBA6 (UBE1L2), FLAG-tag

SKU : BHP18200808

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Mfr.No.	80303
Description	Human UBA6, also known as UBE1L2 (GenBank Accession No. NM_018227), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Immunogen Region	2-end
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 80 ug/mlFLAG peptide, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Reaction mixturecontaining UBA6 (UBE1L2) as a positivecontrol, Ubc5a (500 ng), and FLAG-Smurf2 (500 ng) was incubated at 37°Cfor 30 minutes with biotinylated ubiquitin. The reaction mix was incubated withAnti-FLAG for 1 hour, then HRP secondaryAb for 30 min, followed bychemiluminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_018227
Mw(kda)	118 kDa

Synonyms	UBA6, UBE1L2
Uniprot	A0AVT1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Groettrup M. et al., Trends Biochem. Sci. 33 (5), 230-237 (2008). 2. Pelzer C. et al., J. Biol. Chem. 282 (32), 23010-23014 (2007).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200808	80303	100 ug	$319		Add to Cart

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