Human Tyk2 (Tyrosine Kinase 2, also known as JTK1), GenBank Accession No. NM_003331, a.a. 871-1187(end), with N-terminal His-tag, MW=38 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Host cell line: Sf9 cells
Immunogen Region
871-1187(end)
Tag
N-terminal His-tag
Formulation
45 mM Tris, pH 8, 125 mM NaCl, 2.4 mM KCl, 0.045% Tween-20, 100 mM imidazole and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was incubated at 30°C for 30 minutes. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_003331
Mw(kda)
38 kDa
Synonyms
Tyk2, Tyrosine Kinase 2, JTK1
Uniprot
P29597
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Firmbach-Kraft I, et al. (1990). Oncogene 5 (9): 1329-36. 2. Partanen J, et al. (1991Proc. Natl. Acad. Sci. U.S.A. 87 (22): 8913-7. 3. Domanski P, et al. (1995). FEBS Lett. 374 (3): 317-22.