Human TRKB, also known as NTKR2 (Neurotrophic Tyrosine Kinase Receptor, type 2) or BDNF/NT-3 Receptor, GenBank Accession No. NM_001018064, a.a. 456-822(end), with N-terminal GST-tag, MW=67.5 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 0.8 pmol/min/ug
Immunogen Region
456-822(end)
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.