Human TRKA, also known as NTKR1 (Neurotrophic Tyrosine Kinase Receptor, type 1), GenBank Accession No. NM_002529, a.a. 442-796(end), with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 0.9 pmole/min/ug
Immunogen Region
442-796(end)
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_002529
Mw(kda)
65 kDa
Synonyms
TRKA, NTKR1, Neurotrophic Tyrosine Kinase Receptor, type 1
Uniprot
P04629
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Koch, A., et al. (2000). FEBS Lett. 469 (1): 72-62. 2. Wiesmann, C., et al. (1999). Nature 401 (6749): 184-83. 3. Yamashita, H. et al. (1999). J. Biol. Chem. 274 (21): 15059-65