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Home / Proteins & Peptide

TPH1, His-tag

SKU : BHP18200626

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BPS Bioscience

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Mfr.No.	71192
Description	Human tryptophan 5-hydroxylase 1 (TPH1) also known asTRPH, GenBank Accession No.NM_004179, a.a. 105-420 with an N-terminalHis-tag, MW = 37 kDa, expressedin an E. coli cell expression system.
Immunogen Region	105-420
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 20% glycerol, 3 mM DTT and170 mM imidazole.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction wasperformed at room temperature in assaybuffer containing 50 mM MOPS, pH 7.0,60 uM tryptophan, 100 mM ammoniumsulfate, 100 uM ferrous ammonium sulfate,0.5 mM TCEP, 0.3 mM 6-methyltetrahydropterin, 0.05 mg/ml catalase, 1mM DTT and TPH1. Fluorescence signalwas measured at Em360nm/Ex300nm. Avoidfreeze/thaw cycles. Storing dilutedenzyme is not recommended, ifnecessary, use carrier protein (BSA 0.1- 0.5%).
GenBank	NM_004179
Mw(kda)	37 kDa

Synonyms	tryptophan 5-hyrdoxylase, TRPH
Uniprot	P17752

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Cote, F., et al., Proc. Nat. Acad. Sci. USA. 2003; 100(23): 13525-13530. 2. Walther, D.J., et al., Science. 2003; 299: 76.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200626	71192	50 ug	$368.50		Add to Cart

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