Recombinant human TNIK (TRAF2 and NCK interacting kinase), encompassing amino acids, 2-325. This construct contains an N-Terminal GST-tag. A short peptide linker placed between GST and the protein includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified. Constructs: TNIK (GST-2-325)
host species: insect
Host species: insect
Host cell line: Sf9 cells
Specific activity: 301 pmol/min/ug
Immunogen Region
2-325
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, and 2 mM glutathione
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done according to TNIK Kinase Assay Kit (Biohippo #78370) with various amounts of TNIK.
Avoid freeze/thaw cycles.
GenBank
NM_015028
Mw(kda)
63 kDa
Synonyms
TRAF2 and NCK-interacting protein kinase, KIAA0551