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Home / Proteins & Peptide

TNF-alpha, Mouse (P. pastoris-expressed)

SKU : BHP18500142

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iXCells Biotechnologies

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Mfr.No.	IX02918, IX02918-1, IX02918-100, IX02918-20
Description	Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-alpha), mouse Tumor Necrosis Factor-alpha (mTNF-alpha) is a cytokine mainly expressed by immune cells (1). A type II transmembrane protein, TNF-alpha is further proteolytically processed to a soluble form (2). The trimeric active TNF-alpha(3) then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 (4). Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	17kDa, observed by reducing SDS-PAGE.
Source	P. pastoris
Host	Mouse
Endotoxin Level	1 EU/ug, determined by LAL method.
Bioactivity	ED500.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of 1 x 108units/mg.

Synonyms	TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2
Sequence	SQNSSDKPVA HVVANHQVEE QLEWLSQRAN ALLANGMDLKDNQLVVPADG LYLVYSQVLF KGQGCPDYVL LTHTVSRFAISYQEKVNLLS AVKSPCPKDT PEGAELKPWY EPIYLGGVFQLEKGDQLSAE VNLPKYLDFA ESGQVYFGVI AL

Purity	95% as analyzed by reducing SDS-PAGE.
Storage	Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 1 week at 4°C or up to 2 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500142-1	IX02918-1	1mg	$1498		Add to Cart
BHP18500142-2	IX02918-100	100ug	$324		Add to Cart
BHP18500142-3	IX02918-20	20ug	$107		Add to Cart

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