Human TIM-3, also known as T-cellimmunoglobulin mucin receptor 3, T-cellmembrane protein 3, TIMD-3, Hepatitis Avirus cellular receptor 2, HAVCR-2, and KIM-3. GenBank Accession No. NM_032782,a.a. 22-200 with a C-terminal Avi-biotin, Histag,expressed in a HEK293 cell expressionsystem. MW = 23.3 kDa. This protein isenzymatically biotinylated using Avi-Tagtechnology. Biotinylation is confirmed to begreater than 90%. This protein runs at ahigher MW by SDS-PAGE due toglycosylation.
Immunogen Region
22-200
Tag
C-terminal Avi-biotin, His-tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Notes
The aggregation state of this product has been confirmed to be<5%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_032782
Mw(kda)
23.3 kDa
Synonyms
T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, HAVCR-2, and KIM- 3
Uniprot
Q8TDQ0
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Zhu, C. et al., Nat. Immunol. 2005;6:1245-1252. 2. Anderson, A.C. et al., Science. 2007;318(5853):1141-1143.