Human T-cell immunoreceptor with Ig andITIM domains (TIGIT), also known as V-setand immunoglobulin domain-containingprotein 9, VSIG9, V-set and transmembranedomain-containing protein 3, and VSTM3,GenBank Accession No. NM_173799, a.a.22-141 fused to Fc region of human IgG,expressed in a HEK293 cell expressionsystem. MW = 39.7 kDa. This protein runsat a higher MW by SDS-PAGE due toglycosylation.
Immunogen Region
22-141
Tag
C-terminal Fc fusion (Human IgG1)
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Human
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Endotoxin Level
<1 EU/ug
Notes
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_173799
Mw(kda)
39.7 kDa
Synonyms
T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3
Uniprot
Q495A1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57. 2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.