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Home / Proteins & Peptide

TIGIT, Fc fusion, Biotin-labeled

SKU : BHP18201619

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Mfr.No.	71251-1
Description	Human T-cell immunoreceptorwith Ig and ITIM domains (TIGIT), alsoknown as V-set and immunoglobulindomain-containing protein 9 (VSIG9), andV-set and transmembrane domaincontainingprotein 3 (VSTM3), GenBankAccession No. NM_173799, a.a. 22-141fused to Fc region of human IgG1 andenzymatically biotinylated using AviTagtechnology, expressed in a HEK293 cellexpression system. MW = 41.6 kDa(monomer). This protein runs at a higherMW by SDS-PAGE due to glycosylation.
Immunogen Region	22-141
Tag	C-terminal Fc fusion (IgG1), AviTag
Formulation	8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, and 20%glycerol
Species	Human
Application	Useful for studying protein binding and for screening small molecules and antibodies.
Notes	100 nM of biotinylatedTIGIT was incubated for 1 hour with theindicated concentration of CD155 (Cat.#71181) in a 10 ul reaction in with TIGITAssay Buffer. Chelate Acceptor beads wereadded and incubated for 30 min. followed byStrep-donor beads for 30 min and alphacounts were read. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_173799
Mw(kda)	41.6 kDa

Synonyms	T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9 (VSIG9), and V-set and transmembrane domaincontaining protein 3 (VSTM3)
Uniprot	Q495A1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57. 2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201619	71251-1	25 ug	$324.50		Add to Cart

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