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Home / Proteins & Peptide

Tie2, His-tag

SKU : BHP18200212

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BPS Bioscience

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Mfr.No.	40270
Description	Human Tie2 (GenBank Accession No. NM_000459), a.a. 771-end with N-terminal His-tag, MW=41 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 140 U/ug Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C.
Immunogen Region	771-end
Tag	N-terminal His-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant Tie2. Avoid freeze/thaw cycles.
GenBank	NM_000459
Mw(kda)	41 kDa

Synonyms	TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1
Uniprot	Q02763

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Ramsden, J.D., et al., Endocrinology 146 (7), 2895-2902 (2005). 2. Tammela,T., et al., Blood 105 (12), 4642-4648 (2005).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200212	40270	10 ug	$390.50		Add to Cart

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