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Home / Proteins & Peptide

Tie2, GST-tag

SKU : BHP18200229

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BPS Bioscience

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Mfr.No.	40370
Description	Human TEK tyrosine kinase, endothelial, also known as Tie2, (GenBank Accession No. NM_000459) a.a. 771-1124(end) with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 1400 pmol/min/ug.
Immunogen Region	771-1124(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, 2 uM substrate (Tyr Peptide 5, Z-lyte kinase assay kit from Invitrogen), and enzyme at 37°C for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_000459
Mw(kda)	66 kDa

Synonyms	TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1
Uniprot	Q02763

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Murray, B.W., et al., Biochemistry. 2001 Aug 28,40(34):10243-53. 2. Yuan, H.T., et al., Mol Cell Biol. 2009 Apr,29(8):2011-22.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200229	40370	10 ug	$390.50		Add to Cart

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