Human TGFb1 (GenBank Accession #NM_000660), a.a. 279-390(end), disulfide-linked homodimer. MW= ~25 kDa. Ths is the mature active form of the protein.
host species: human
Host species: human
Host cell line: FreeStyle 293-F cells
Reconstitution
Reconstitute in sterile 0.2 M acetic acid with 0.1% BSA. Further dilutions may be made in PBS with 0.1% BSA. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Immunogen Region
279-390(end)
Tag
none
Formulation
Lyophilized from a 0.2 um filtered, aqueous solution
Species
Human
Application
Useful for cell culture and for the study of signaling and apoptotic pathways.
Endotoxin Level
< 1EU per ug
Notes
Avoid freeze/thaw cycles.
Biological Activity Comment
The ED50 measured by TGFbeta1's ability to inhibit mIL-4-dependent proliferation of HT-2 mouse helper T cells is<0.05ng/ml.