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Home / Proteins & Peptide

TET1, FLAG-tag

SKU : BHP18201504

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BPS Bioscience

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Mfr.No.	50161-1
Immunogen Region	1418-2136
Tag	N-terminal FLAG-tag
Description	Human Ten-eleven translocation 1protein (TET1), methylcytosinedioxygenase TET1, Leukemiaassociatedprotein with a CXXC domain,CXXC-type zinc finger protein 6,GenBank Accession # NM_030625, a.a.1418-2136(end) with N-terminal FLAG-tag,MW = 80 kDa, expressed in Sf9cells via a baculovirus expressionsystem.
Formulation	40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	TET enzyme wascombined with a TET methylatedsubstratein assay buffer for 90 minutes.A fluorescent-conjugated antibody thatrecognizes hydroxymethylated productwas added and fluorescence was read atexcitation: 530 nm and emission 590 nm. Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank	NM_030625
Mw(kda)	80 kDa

Synonyms	Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6
Uniprot	Q8NFU7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tahiliani, M., et al., Science. 2009; 324(5929): 930-935. 2. Guo, J.U., et al., Cell. 2011; 145(3): 423- 434.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201504	50161-1	10 ug	$286		Add to Cart

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