Human tyrosyl DNA phosphodiesterase 2, or TDP2, (GenBank Accession No. NM_016614), full length with N-terminal His-GST-tag, MW = 67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Specific activity: >= 186 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal His-GST-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol and 3 mM DTT
Species
Human
Notes
Enzyme reaction was performed in areaction buffer (200 ul) containing 50 mMTris (pH 7.4), 1 mM MgCl2, 50 mM KCl, 1mM DTT, 0.1 mg/ml BSA, 2 mM T5PNP and600 ng TDP2. Reaction was continuouslymonitored at 415 nm at room temperature for20 min. Specific activity was calculated based on themolar extinction coefficient of the product (p-nitrophenol),e = 15,000 M-1cm-1
Avoid freeze/thaw cycles.
GenBank
NM_016614
Mw(kda)
67 kDa
Synonyms
PDE, tyrosyl DNA phosphodiesterase2
Uniprot
O95551
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Loughney, K, et al., Int. J. Impot. Res.2005, 17(4):320-5 2. Weeks, JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9