Human Tryptophan 2,3-dioxygenase (TDO), also known as TDO2, GenBank Accession No. NM_005651, a.a. 2-406(end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 49 kDa.
Immunogen Region
2-406(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, and 300 mMimidazole
Species
Human
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
Assay was performed following the BPS TDO Inhibitor Screening Assay Kit (Cat. #72023) and following the protocol provided with the kit.
Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
GenBank
NM_005651
Mw(kda)
49 kDa
Synonyms
Tryptophan 2,3-dioxygenase, TDO2, TDO-2
Uniprot
P48775
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Pillotte, L., et al., Proc. Nat. Acad. Sci (USA). 2012; 109(7):2497-2502. 2. Miller, C.L., et al., Neurobiol. Disease. 2004; 15(3): 618-629.