TNKS2 (TRF1-interacting ankyrin-related ADP-ribose polymerase 2), also known as PARP5B, tanykyrase-2, or TNKL (GenBank Accession No. NM_025235), a.a. 849-1166(end), with N-terminal GST-tag, MW=62kDa, expressed in Sf9 cells via a baculovirus expression system.
Unit definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Immunogen Region
849-1166(end)
Tag
N-terminal His-GST-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16mM glutathione, 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted using BPS's TNKS2 HistoneRibosylation Assay Kit (Biotin-labeled NAD+),Catalog # 80578.