TNKS1 (TRF1-interacting ankyrin-related ADP-ribose polymerase 1), also known as PARP5A, TANK1, or tanykyrase-1 (GenBank Accession No. NM_003747), a.a. 1001-1327(end), with N-terminal GST-tag, MW=62.5 kDa, expressed in Sf9 cells via a baculovirus expression system.
Unit definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Immunogen Region
1001-1327(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted using BPS's TNKS1 HistoneRibosylation Assay Kit (Biotin-labeled NAD+),Catalog # 80573.
1. Chang S. (2010). Aging (Albany NY). 2(10):639-42. 2. Karner, C.M. et al. (2010). Dev Dyn. 239(7):2014-23. 3. McCabe, N, et al. (2009). Oncogene. 28(11):1465-70.