JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

TAK1-TAB1, GST-tag

SKU : BHP18200213

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40279
Description	Recombinant fusion protein consisting of human proteins TAK1 (TGFbeta-activated kinase 1), also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7), GenbankAccession #NM_003188, a.a. 1-303, and human TAB1 (TAK1-binding protein), Genbank Accession #NM_006116, a.a. 437-end, linked by a small peptide (DFGGGGG), and with an N-terminal GST-tag, MW = 74 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells
Background	TAK1 is a serine/threonine protein kinase that mediates signaling by TGFbeta and morphogenetic protein (BMP) (1). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (Nf B). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate Nf B and JNK and are prone to apoptosis upon stimulation.
Immunogen Region	TAK1 (1-303), TAB1 (437-end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in a kinase buffer containing 0.2 mM DTT using MBP substrate (0.1 mg/ml) and 100 mM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using ADP-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_003188, NM_006116
Mw(kda)	74 kDa

Synonyms	TAK1: MAP3K7, TGF1a, TGFbeta-activated kinase 1 TAB1: MAP3K7IP1, 3'-Tab1, MGC57664, TAK1-binding protein
Uniprot	O43318(TAK1), Q15750 (TAB1)

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1.Yamaguchi, K. et al. Science 270: 2008-2011, 1995. 2.Liu, H.-H. et al. Proc. Nat. Acad. Sci. 103: 11677-11682, 2006. 3. Sakurai, H., et al. (2000). FEBS Lett.(Netherlands) 474 (2-3): 141-5. 4. Liu, H.-H., et al. (2006). Proc. Natl. Acad. Sci. USA 103: 11677-11682.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18200213	40279	10 ug	$423.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.