Human TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, also known as TAF1L, GenBank Accession No. NM_153809, a.a. 1398 - 1649 corresponding to bromodomains 1 and 2 with N-terminal GST-tag, MW = 56.1 kDa, expressed in an E. coli expression system
Immunogen Region
1398-1649
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glyercol
Species
Human
Application
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes
TAF1L (1398-1649) protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in TAF1L assay buffer in a 10 ul reaction for 1 hour at RT. AlphaLISA GSH acceptor beads (PerkinElmer) are added, and the mixture is incubated for 30 minutes at room temperature (RT). AlphaScreen Streptavidin-conjugated donor beads (PerkinElmer) are then added. After 10 minute incubation at RT, measure Alphacounts.
Avoid freeze/thaw cycles.
GenBank
NM_153809
Mw(kda)
56.1 kDa
Synonyms
TAF1 RNA polymerase II, TAT box binding protein (TBP)-associated factor, TAF1L