T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA beta-glucosyltransferase, Genbank Accession No. NP_049658, a.a. 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.
Immunogen Region
1-351(end)
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species
T4 bacteriophage
Application
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.