Recombinant full-length human SYK (GenBank accession #NM_003177) with an N-terminal GST-tag was expressed in Sf9 insect cells via a baculovirus expression system. MW = 10 kDa.
Host cell line: Sf9 cells
Specific activity: 95 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione,3 mM DTT and 10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Avoid freeze/thaw cycles.
GenBank
NM_003177
Mw(kda)
100 kDa
Synonyms
SYK, Spleen Tyrosine Kinase
Uniprot
P43405
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41. 2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.