Recombinant human SYK, catalytic subunit (GenBank Accession # NM_003177), a.a. 359-635(end) with an N-terminal GST-tag, was expressed in Sf9 insect cells using a baculovirus expression system. MW = 58 kDa.
Host cell line: Sf9 cells
Specific activity: 139 pmol/min/ug
Immunogen Region
359-635(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
SYK was incubated with a substrate (Tyr peptide 2) for 1 hour at RT in 1X kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.
Avoid freeze/thaw cycles.
GenBank
NM_003177
Mw(kda)
58 kDa
Synonyms
SYK, Spleen Tyrosine Kinase
Uniprot
P43405
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Takano, T. et al. Antioxid Redox Signal. 2002 Jun,4(3):533-41. 2. Navara, C S. Curr Pharm Des. 2004,10(15):1739-44.