Human SUZ12 (suppressor of zeste 12), Genbank Accession #NM_015355, (a.a. 2-739 (end)) with N-terminal His and FLAG-tags, MW = 85 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Host cell line: Sf9 cells
Immunogen Region
2-739 (end)
Tag
N-terminal His-FLAG-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 20% glycerol, 1 mM DTT, and 100 ug/ml FLAG peptide
Species
Human
Application
Useful for binding studies to investigate protein-protein interactions in the EZH2 complex. Also suitable as an immunogen or as a standard for Western blotting.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_015355
Mw(kda)
85 kDa
Synonyms
Suppressor Of Zeste 12 Homolog, CHET9, JJAZ1
Uniprot
Q15022
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Cao, R, Zhang, Y. Mol Cell. 15(1): 57-67 (2004). 2. Brecqueville, M, et al. Blood Cancer J. 1(8):e33 (2011). 3. Martin-Perez, D., et al. Am J Pathol. 2010 Aug;177(2):930-42