Human SUV39H2, GenBank Accession No. NM_024670, a.a. 26-350, with N-terminal GST-tag, MW= 63 kDa, expressed in an E. coli expression system.
Host cell line: E. coli
Immunogen Region
26-350
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 20 uM S-adenosylmethionine, and 0-40 ng SUV39H2) is added to the wells of a substrate-coated plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr.Finally, incubate 1 hr. with the Europium-labeled secondaryantibody. Time resolved fluorescencemeasured at room temperature ( exc=330nm, em=620 nm).
Avoid freeze/thaw cycles.
GenBank
NM_024670
Mw(kda)
63 kDa
Synonyms
KMT1B
Uniprot
Q9H5I1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Yoon,K.A. et al., Carcinogenesis 27, 11, 2217-2222, 2006. 2. Frontelo, P. et al., Oncogene 23, 30, 5242- 5251, 2004.