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Home / Proteins & Peptide

SUV39H1, GST-tag

SKU : BHP18200434

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BPS Bioscience

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Mfr.No.	51070
Description	Human SUV39H1, GenBank Accession No. NM_003173, a.a. 82-412, with N-terminal GST-tag, MW= 64 kDa, expressed in an E. coli expression system. Host cell line: E. coli Specific activity: >=0.1 pmol/min/ug
Immunogen Region	82-412
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 uM S-adenosylmethionine, and 10-100 ng SUV39H1) is added to the wells of a substrate-coated Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_003173
Mw(kda)	64 kDa

Synonyms	KMT1A
Uniprot	O43463

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kang, M.Y. et al., Int. J. Cancer 121, 10, 2192-2197, 2007. 2. Spensberger, D. and Delwel, R. FEBS Lett. 582, 18, 2761-2767, 2008.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200434	51070	50 ug	$500.50		Add to Cart

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