Human SRC (GenBank Accession No. NM_005417), full length with N-terminal His-tag, MW = 60.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 205 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200 uM ATP, 2 uM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.