Severe acute respiratory Coronavirus Spike trimer (S1+S2), Genbank Accession No. MN908947, a.a. 16-1213, with a with C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=139 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Immunogen Region
16-1213
Tag
C-terminal His-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole and 20% glycerol.
Species
SARS-CoV-2
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
SARS-CoV2-Trimer Eu-labeled protein (6.25 nM) was incubated with ACE2, His-Avi-Tag, Biotin-labeled HiP (Biohippo, #100665) and the Dye-labeled acceptor in ACE2-Spike TR-FRET buffer for 1 hour at room temperature. TR-FRET signals were measured and the ratio was calculated as (Ex340nm/Em665nm) /(Ex340nm/Em620nm).
Avoid freeze/thaw cycles.
GenBank
MN908947
Mw(kda)
139 kDa +glycans
Synonyms
covid, covid-19, covid19, coronavirus, Severe acute respiratory Coronavirus Spike trimer, SARS Cov-2 mutant, S protein, Spike S1, Spike S2, ncov