Recombinant SARS-CoV-2 Spike protein in its homotrimeric form, containing S1+S2 subunits and encompassing amino acids 16-1213. This protein corresponds to SARS-CoV-2 Variant B.1.617.1 also known as variant Kappa originally identified in India, and contains mutations G142D, E154K, L452R, E484Q, D614G, P681R and Q1071H. The construct also contains mutations 682RRAR685>A, K986P and V987P, and a T4 trimerization domain followed by a His-tag (6xHis) in C-terminal. The recombinant protein was affinity purified. Constructs: Spike Trimer (S1+S2) (B.1.617.1 Variant) (16-1213-His) (SARS-CoV-2)
Immunogen Region
16-1213
Tag
C-terminal His-tag
Formulation
8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Mutation
G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H
Species
SARS-CoV-2
Notes
Avoid freeze/thaw cycles.
Mw(kda)
137 kDa + glycans
Synonyms
coronavirus, covid, covid-19, covid19, Sars-cov-2 p1, sars cov 2 Spike, sarscov2, SARS-CoV-2 variant b16171, Lineage B.1.617.1, B16171 mutation, India coronavirus, SARS-CoV-2 India, India, India variant, kappa, Kappa variant, 101144-1, 101144-2