Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 13-665, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 expression system, MW=102 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
13-665
Tag
C-terminal Avi-tag
Formulation
8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
SARS-CoV-2
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
SARS-CoV-2 S1 and ACE2 binding assay was done using BPS ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (Biohippo #79936). SARS-CoV-2 S1 protein and anti-human Fc-HRP were used instead of SARS-CoV-2 Spike protein (RBD) and anti-mouse Fc-HRP respectively. Non-specific His-tag protein was coated and used for a negative control.
Avoid freeze/thaw cycles.
GenBank
QHD43416.1
Mw(kda)
102 kDa
Synonyms
SARS-CoV s1, coronavirus spike S1,
Uniprot
P0DTC2 SPIKE_WCPV
Shipping Temperature
-80°C (dry ice)
Storage
At least 6 months at -80°C.
Reference
1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10. 2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12