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Home / Proteins & Peptide

Spike S1 (13-665), Fc fusion, Avi-tag, Biotin-Labeled (SARS-CoV-2)

SKU : BHP18201234

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BPS Bioscience

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Mfr.No.	100679-2
Description	Human Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1 (SARS-CoV-2 Spike S1), GenBank Accession No. QHD43416.1, a.a. 13-665, fused at the C-terminus of the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 expression system and enzymatically biotinylated using Avi-tag technology. Biotinylation is confirmed to be>=90%. MW=102 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region	13-665
Tag	C-terminal Fc-Avi-tag
Formulation	8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Nickel plate wells were coated with 1 ug/ml ACE2, His-Tag (Biohippo, #11003, 50 ng/well). Then, 2019-nCoV Spike S1, Fc-fusion (IgG1), Biotin-labeled was incubated with ACE2, His-tag for 1 hour at room temperature, 50ul/well. Bound protein was incubated with Streptavidin-HRP (Biohippo, #79742) and luminescence was measured in a BioTek Synergy 2 microplate reader. Avoid freeze/thaw cycles.
GenBank	QHD43416.1
Mw(kda)	102 kDa

Synonyms	Severe acute respiratory Coronavirus 2 Spike Glycoprotein S1
Uniprot	P0DTC2 SPIKE_WCPV

Shipping Temperature	-80°C (dry ice)
Storage	At least 6 months at -80°C.

Reference	1. Hoffman, M. et al., Cell. 2020 Apr; 181:1-10. 2. Walls, A. C. et al., Cell. 2002 Mar; 180:1-12

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201234	100679-2	50 ug	$495		Add to Cart

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