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Home / Proteins & Peptide

Sphingosine kinase 2 (long), His-tag

SKU : BHP18200274

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BPS Bioscience

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Mfr.No.	40612
Description	Human Sphingosine kinase 2 (GenBank Accession No.NM_020126), full length with N-terminal His-tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system Host cell line: Sf9 cells Specific activity: 20 U/ug Unit definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.75 ug/ml sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_020126
Mw(kda)	74 kDa

Synonyms	SphK2, sphingosine kinase 2, SPK2
Uniprot	P08571

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	Okada, et al., J. Biol. Chem. 280: 36318-36325 (2005).Application Reference: Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200274	40612	10 ug	$456.50		Add to Cart

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