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Home / Proteins & Peptide

Sphingosine kinase 2, His-tag

SKU : BHP18200273

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Mfr.No.	40611
Description	Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His-tag, MW=69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 20 U/ug Unit definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.75 ug/ml sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	AF245447
Mw(kda)	69.5 kDa

Synonyms	SphK2, sphingosine kinase 2, SPK2
Uniprot	Q9NRA0

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Zemann, B., et al., Blood.107(4):1454-8 (2006) 2. Maceyka, M., et al., J Biol Chem. 280(44):37118-29 (2005)Application Reference: 1.Synthesis and biological evaluation of carbocyclic analogues of pachastrissamine (2015) 2.Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200273	40611	20 ug	$456.50		Add to Cart

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