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Home / Proteins & Peptide

Sphingosine kinase 1, His-tag

SKU : BHP18200272

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BPS Bioscience

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Mfr.No.	40610
Description	Human Sphingosine kinase 1 or SphK1 (GenBank Accession No. AF200328), full length with N-terminal His-tag, MW=46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 1900 pmole/min/ug Unit definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 20 uM Sphingosine and 20 uM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Assay was done in Kinase buffer (40 mM Tris, pH 7.4, 20 mM MgCl2+, 0.1 mg/ml BSA) containing 20 uM Sphingosine and 20 uM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) Avoid freeze/thaw cycles.
GenBank	NM_001142601.1
Mw(kda)	46.9 kDa

Synonyms	SphK1, sphingosine kinase 1, SPK1
Uniprot	Q9NYA1-1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004). 2. Taha, TA., et al., FASEB J. 20(3):482-4 (2005).Application Reference:Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200272	40610	20 ug	$445.50		Add to Cart

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