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Home / Proteins & Peptide

SMYD4, FLAG-tag

SKU : BHP18200432

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BPS Bioscience

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Mfr.No.	51057
Description	Human SET and MYNDcontainingprotein 4 (SMYD4), alsoknown as KIAA1936, amino acids 2-804(end), with N-terminal FLAG-tagGenBank Accession No. NM_052928,MW = 90 kDa. This protein is expressedin a Baculovirus infected Sf9 cellexpression system. host species: insect Host species: insect Host cell line: Sf9
Immunogen Region	2-804(end)
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, 80 ug/mlFLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Add 50 ul reaction mix (100 uM S-adenosylmethionine, SMYD4 enzyme, and HMT buffer 7Z' (BPS #52193Z) to wells coated with the substrate. Incubate 3-16 h. Add antibody against methylated K4 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended.
GenBank	NM_052928
Mw(kda)	90 kDa

Synonyms	SET and MYND domain-containing protein 4, KIAA1936
Uniprot	Q8IYR2

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200432	51057	20 ug	$484		Add to Cart

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