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Home / Proteins & Peptide

SMURF1, FLAG-tag

SKU : BHP18200830

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BPS Bioscience

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Mfr.No.	80402
Description	Human SMURF1 (GenBank Accession No. NM_020429), a.a. 150-end, with N-terminal FLAG-tag, MW= 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Immunogen Region	150-end
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, 3 mM DTT, 20% glycerol
Species	Human
Application	Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	50 ul of reaction mix (4 mM ATP, 5 mM MgCl₂, 1 mM DTT, E1 (0.5 ug), E2 (2.8 ug), Biotin-Ubiquitin (0.5 ug) and SMURF1 (0-1.25ug) in 50 mM Tris pH 7.4) was incubated for 30 min at 37°C. The reaction mixture was transferred to a streptavidin-coated plate and incubated for additional 30 min at 37°C. Anti-SMURF1 and HRP-labeled secondary antibody were incubated with the reaction mixture for 1 hour each, followed by luminescent detection. Avoid freeze/thaw cycles.
GenBank	NM_020429
Mw(kda)	70 kDa

Synonyms	SMAD specific E3 ubiquitin protein ligase 1, SMURF1
Uniprot	Q9HCE7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Narimatsu M. et al., Cell 137 (2), 295-307 (2009) 2. Fukunaga E. et al., J. Biol. Chem. 283 (51), 35660-35667 (2008).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200830	80402	20 ug	$429		Add to Cart

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