Human Bromodomain SMARCA2 or BRM, a.a. 1375 - 1511 (GenBank Accession No. NM_003070) with N-terminal Avi-His-tag, MW = 19 kDa, expressed in an E. coli expression system. This protein is enzymatically biotinylated using Avi-Tag technology. Biotinylation is confirmed to be>=90%
Immunogen Region
1375-1511
Tag
N-terminal Avi-His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.
Species
Human
Application
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_003070
Mw(kda)
19 kDa
Synonyms
SMARCA2, BRM
Uniprot
P51531
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Reference
1. Shain, A.H., et al., Proc Natl Acad Sci USA. 2012 Jan 31; 109(5): E252-9.2. Liu, G., et al., Oncogene. 2011 Jul 21; 30(29): 3295-304.