Human SMARCA2, also known as BRM, GenBank Accession No. NM_003070, a.a. 1375-1511 corresponding to single bromodomain, with N-terminal GST-tag, MW=43kDa, expressed in expressed in an E. coli expression system.
Immunogen Region
1375-1511
Tag
N-terminal GST-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Notes
SMARCA2 is incubated with Bromodomain Ligand 2 in SMARCA2 buffer in a 10 ul reaction for 30 minutes. AlphaLISA GSH acceptor beads (PerkinElmer) are added, followed by AlphaScreen Streptavidin-conjugated donor beads (PerkinElmer). After 10 minutes incubation at RT, Alphacounts are measured.