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Home / Proteins & Peptide

Sirtuin 5, GST-tag

SKU : BHP18200313

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BPS Bioscience

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Mfr.No.	50016
Description	Human Sirtuin 5 (GenBank Accession No. NM_012241), full length with N-terminal GST-tag, MW= 59.8 kDa, expressed in an E. coli expression system. Specific activity: >=2.0 pmole/min/ug
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, and 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, 0.1 mg/ml BSA, 200 uM SIRT5 substrate 5 (Catalog #50401), 500 uM NAD+ and Sirtuin 5 (15.6 - 1000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 ul of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460. Avoid freeze/thaw cycles.
GenBank	NM_012241
Mw(kda)	59.8 kDa

Synonyms	Sirtuin5, SIRT5
Uniprot	Q9NXA8

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Mahlknecht, U. et al., (2006) Cytogenet.Genome Res. 112, 208-212. 2. Frye, R.A. (2000) Biochem. Biophys. Res.Commun. 273, 793-798.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200313	50016	100 ug	$484		Add to Cart

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