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Home / Proteins & Peptide

Sirtuin 3, GST-tag

SKU : BHP18200311

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BPS Bioscience

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Mfr.No.	50014
Description	Human Sirtuin 3 (GenBank Accession No. NM_012239), a.a. 102-399 with N-terminal GST-tag, MW= 59 kDa, expressed in an E. coli expression system. Specific activity: >=8.5 pmole/min/ug Unit definition: One U =1 pmol of acetyl group removed/min/ug of enzyme
Immunogen Region	102-399
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 uM HDAC substrate 1 (Catalog #50032), 500 uM NAD+ and Sirtuin 3 (31 - 2000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 ul of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460. Avoid freeze/thaw cycles.
GenBank	NM_012239
Mw(kda)	59 kDa

Synonyms	Sirtuin3, SIRT3
Uniprot	Q9NTG7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Scher, M.B. et al., Genes Dev. 21 (8), 920-928 (2007) 2. Bellizzi, D. et al., Genomics 89 (1), 143-150 (2007)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200311	50014	100 ug	$484		Add to Cart

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