Human Sirtuin 2, GenBank Accession No. NM_012237, a.a. 50-356 with C-terminal His-tag, MW= 35.5 kDa, expressed in an E. coli expression system.
Specific activity: >=1.0 pmol/min/ug.
Immunogen Region
13-319
Tag
C-terminal His-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.1 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 1 (Catalog #50032), 500 uM NAD+ and Sirtuin 2 (125 - 8000 ng). Incubation condition: 30 min at 37°C followed bytreatment with 50 ul of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Avoid freeze/thaw cycles.
GenBank
NM_012237
Mw(kda)
35.5 kDa
Synonyms
Sirtuin2, SIRT2
Uniprot
Q8IXJ6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Grubisha, O., 2006, J.Biol.Chem. 281, 20, 14057-65. 2. Vaquero, A., 2006, Genes Dev. 20, 10, 1256- 61.Product Specific Reference(s): 1. Nguyen, P., 2014, Mol Cancer Res. 12, 9, 1244-53.