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Home / Proteins & Peptide

Sirtuin 1 (193-741), GST-tag

SKU : BHP18200309

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BPS Bioscience

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Mfr.No.	50012
Description	Human Sirtuin 1 , GenBank Accession No. NM_012238, a.a. 193-741 with N-terminal GST-tag, MW= 87.2 kDa, expressed in an E. coli expression system. Specific activity: >=20 pmol/min /ug
Immunogen Region	193- 741
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 1 (Catalog #50032), 500 uM NAD+ and Sirtuin 1 (12.5 - 800 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 ul of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_012238
Mw(kda)	87.2 kDa

Synonyms	Sirtuin1, SIRT1, Sir2, Sir2a
Uniprot	Q96EB6

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kolthur-Seetharam, U. et al (2006) Cell Cycle 5, 873-877. 2. Solomon, J.M. et al (2006) Mol. Cell. Biol. 26, 28-38. Application References 1. Arcand, M., et al. "Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases." Posterpresented as part of the BioTechniques Fall 2011 Epigenetics Meeting.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200309	50012	100 ug	$484		Add to Cart

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