Signal regulatory proteinalpha (SIRP-alpha), also known as Tyrosineprotein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1, GenBank Accession No. NP_542970, a.a. 27-370, with C-terminal Avi and His tags, MW 40.3 kDa*, expressed in a HEK293 expression system and enzymatically biotinylated using Avi-tag technology. This protein acts as a receptor for CD47; a.a. 27-370 correspond to the extracellular portion of SIRP-alpha. *Note: SIRP-alpha is heavily glycosylated, resulting in higher molecular weight.
Immunogen Region
27-370
Tag
C-terminal His-Avi-Tags
Formulation
8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Species
HEK293
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NP_542970
Mw(kda)
40.3 kDa
Synonyms
Tyrosine-protein phosphatase non-receptor type substrate 1, CD172a, Bit, and MyD-1
Uniprot
P78324
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Hatherley, D., et al., Mol. Cell, 2008, 31:266-77 2. Baba, N., et al., J. Exp. Med., 2013, 210(6):1251-63